"Our priorities and stages of life are different now, and so we decided that this is the best time to end our partnership."
Williams, 50, has long hinted at retirement to spend more time in New Zealand with his wife and eight-year-old son but was hoping to continue with Scott next year from March to September, almost all of the Australian's playing schedule.
"My initial plan for the future was to reduce the amount of tournaments I caddied in," Williams said.
"After discussing this in detail with Adam it became evident that my plan was not going to fit with Adam's requirements and so we decided to end our partnership.
"Having caddied for the first Australian to win the Masters is a career highlight and a memory I will cherish forever.
"If the right opportunity arose I would consider caddying on a part-time basis in the future."
In three seasons together the pair won the 2011 World Golf Championship Bridgestone Invitational, the Australian Masters in 2012 and 2013, the Barclays in 2013, the Australian PGA Championship in 2013, the Crowne Plaza Invitational in 2014 and his first major championship, the Masters in 2013.
Scott also spent 11 weeks as world No.1 earlier this year, just the second Australian to reach the milestone behind Norman.
Scott's next event is in Japan next month before he heads to Australia for the triple crown in November and December.
- AAP