Phil Rawlins, president of Orlando City Soccer Club, said at the unveiling of the rainbow tribute: "These are regular season-tickeholder seats.
"We put them in Section 12 obviously because we felt that was pertinent - it was June 12 last year when the tragedy happened. So they're right here in Section 12, they're right down by the benches.
"They'll certainly be seen by everybody inside the stadium, and a very significant reminder of that day."
Orlando have played in the MLS for two seasons at the city's Camping World 'Citrus Bowl' Stadium while their new home was under construction.
The Orlando City Stadium will hold a capacity of 25,500 and has a whole end reserved for safe-standing.