This will mark the first visit to Cincinnati for the USA Women since 2008 and its first match at Nippert Stadium, home to FC Cincinnati of the United Soccer League.
USA head coach Jill Ellis said they are looking forward to taking on a quality opponent.
"The fall friendly matches are an important transition period to 2018 and we want to get the most out of them by continuing to play teams with World Cup and Olympic experience like New Zealand," Ellis said.
"Denver has been a fun place to play for our team over the years, but we also enjoy going to cities where we haven't been in a while, like Cincinnati. The fans there have shown extraordinary support for their USL team and we're looking forward to an enthusiastic crowd for our match as well."
The USA and New Zealand have met 14 times in history, with the World No 1 having won 12, drawn once and lost once to the Ferns.
These international friendlies will be the first meetings with New Zealand on American soil since a 4-0 win in St. Louis on April 4, 2015 in the lead up to the 2015 FIFA Women's World Cup.
The tour will mark 30 years since New Zealand's only victory over the USA which came in the first match between the teams back in 1987, a 1-0 victory for the Football Ferns.
The USA and New Zealand have played once before in Cincinnati, a 6-0 USA win in 2004 on the post-Olympic tour after the USA won the gold medal in Athens, Greece.
Readings will name his Football Ferns squad for the USA Tour on 26 August.
Football Ferns Tour of the USA
Game 1
15 September, kick off at 8pm (local time)
Dick's Sports Goods Park in Commerce City, Colorado
Game 2
19 September, kick off 7:30 pm (local time)
Nippert Stadium on the campus of the University of Cincinnati