Both Neylan and Gillow were prominent in the recent UCI New Zealand Cycle Tour.
The new domestic women's team Soul Star Racing will make their racing debut at the championship led by former New Zealand senior women's road race champion Gabrielle Vermunt.
There's a battle of the trade teams in the elite men's competition with a strong contingent from New Zealand's Subway Pro Cycling led by Paul Odlin and Sam Horgan, who were first and second respectively in the time trial at January's national championships.
They face a battle against UCI Pro Continental team Team Budget Forklifts, who have already won two races this year and are led by the talented Shaun McCarthy.
There are strong numbers in the under-23 and under-19 fields where South Canterbury's Jason Christie, runner-up in the time trial at the national championships, is likely to feature prominently.
Christie was sixth in the under-23 time trial at the world championships last year.
He will face a tough challenge from current Omnium world champion Michael Freiberg (Jayco-AIS) and Campbell Flakemore (Genesys Wealth Advisors) who won the opening time trial of the NZ Cycle Classic earlier this year.
Sophie Williamson, from nearby Alexandra, will lead the way in the junior women's ranks after her top-10 finish at the world championships.
The championships begin with the time trials on Friday with the under-19 women (76.5km) and elite women (102km) road races on Saturday and the under-19 (102km) and both Under-23 and elite men(153km) races on Sunday.
The course is a 25km circuit that starts and finishes at the Nugget Point Resort at Arthurs Point incorporating some challenging climbs near Arrowtown.