Queensland's fielders dropped to the floor, hands on heads in genuine disbelief. Tremain unsurprisingly stood his ground.
With no DRS, HotSpot or Snicko available, viewers from home were puzzled by replays, a somewhat optical illusion from the front-on camera making it impossible to decipher whether the ball came off bat or thigh pad.
Despite the majority of cricketers being certain that the ball had come off the bat, a poll from The Cricketer on Twitter returned 62 per cent of people believing the ball had come off Tremain's pad, leading to Black Caps all-rounder Jimmy Neesham chiming in.
England bowler Stuart Broad also took the opportunity to have a dig.
Tremain's non-wicket was not the first contentious decision of the match — earlier, Victorian opener Eamonn Vines was given out caught behind, with replays suggesting the ball had only brushed his shirt.
With eight deliveries left in the day, legspinner Mitch Swepson finally got the breakthrough to achieve a memorable victory, trapping Holland lbw for a 28-ball duck.
Tremain and Holland lasted 52 deliveries at the crease together without scoring a run.
Swepson finished with match figures of 7-92, including a rare hat-trick in the first innings.