The regional council has contacted businesses between St Andrews and Horotiu that are drawing water immediately to exercise caution and, if concerned, to use alternative water sources until test results are available.
The public is also being urged to be cautious about entering the water immediately downstream too.
The foam has been spotted floating along the river from the St Andrews stormwater pipe to Pukete before dispersing.
While the tests are being carried out, the regional council is making its own inquiries and Hamilton City Council staff are also checking their stormwater network to see if they can identify a possible source for the discharge.