In the last year alone 6000 native trees were planted by volunteers in wetlands and other areas along the trails.
The trust also built 18km of fencing that provides a barrier between farms and the Waikato River.
"Thanks to the unwavering support of our volunteers and the outstanding support of the project partners and land owners adjacent to the trails, the dream of the Waikato River Trails is now becoming a reality," Mr Wooller said.
"The South Waikato community and indeed all New Zealanders can be truly proud of the Waikato River Trails, a permanent new asset that will showcase the best of this beautiful region."
New Zealand Cycle Trail project manager Richard Balm said the Waikato River Trails would achieve several objectives, including boosting tourism opportunities, providing employment, and opening up public access to "the beautiful Waikato River for visitors and locals alike".
"The Waikato River Trails will offer riders and walkers the chance to experience the magic and beauty of native bush, exotic forest, historic landmarks, interesting rock formations, rolling farmland and open reserves, as well as boardwalks through significant wetlands, and expansive lake and river views," he said.