"I'm frankly at a loss at how to otherwise improve the behaviour," he said after ending Question Time at the beginning of the 12th and final question today.
"I am now ruling that Question Time is concluded because I cannot think of a better way of indicating my concern to Mr Jones and other members of the Government about their inappropriate personal comments."
This is the second time in two days the Speaker has disciplined the Government for offensive comments made to Brownlee.
Yesterday, after an interruption from Brownlee, Deputy Prime Minister Winston Peters said: "Ho, ho Billy Bunter" – a reference to the fictional overweight school boy William George "Billy" Bunter.
After ordering Peters to withdraw and apologise, Mallard gave the National Party an additional six supplementary questions.
This is not the first time Peters has insulted Brownlee over his size. Last month, Peters yelled "throw fatty out" at Brownlee, after he interjected during an answers.
But Brownlee is not Peters' only target. During the third reading of the Waka Jumping Bill this afternoon, Peters referred to National MP Maggie Barry as "Myrtle Rust."
Brownlee did not wish to comment on the matter