The litter comprises five males and four females and the puppies are either Staffordshire bull terrier or mastiff crosses - "probably more likely to be a mastiff, because of the size of the litter".
"Someone must know if their neighbour's dog had nine puppies and now doesn't have any, so we're hoping to get a lead," Mrs Saunders said.
Puppies generally were not weaned until eight weeks, so no attempt would be made to find homes for them until then.
Although they were "very cute", it would be hard to place nine puppies at once in Central Otago, so the puppies might end up at the SPCA's Dunedin base.
The cost to the SPCA of each puppy it "rehomed" was $535, so this litter would cost the organisation $4815, she said.
That figure includes the cost of care, feeding, worming, vet care, vaccination, desexing and microchipping.
The SPCA would like to hear from anyone with information on the puppies. They can phone on 0800 682-467 to provide information anonymously.