Protest organiser Genevieve Toop says the group are taking a stand because big new irrigation schemes like Central Plains Water mean more cows, and more cows mean more sick rivers.
The group said it had been encouraged by a recent Supreme Court decision that's halted work on the long-disputed Ruataniwha Dam project, which would have created water for irrigation in the Hawke's Bay.
The Central Plains project is the largest of several irrigation schemes receiving funding from the Ministry for Primary Industries.
The project has a total construction cost of about $385 million and will provide water to about 60,000 hectares on the Canterbury Plains, according to its website.
"This is an enormous publicly funded irrigation scheme which will increase the dairy herd and pollute Canterbury's rivers," Toop said.