But she said only a week ago the zone was lifted from about 24,000ft after a risk assessment of weaponry believed to be on the ground.
That proved inadequate after flight MH17 with 298 people on board was shot down from 33,000ft by what is feared to have been a Buk missile capable of reaching more than 70,000ft.
A global "real time" map of air movements last night showed there were no flights over the disputed territory, although aircraft were plying routes further south over the Black Sea.
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Air traffic agency Eurocontrol said it was now rejecting all flight plans filed using routes over Ukraine, which were closed "until further notice".
The agency said that would affect about 300 flights a day, even though pilots and airlines had previously been warned to avoid Ukrainian airspace.
British aviation security expert Norman Shanks said Malaysia Airlines was among a number of carriers that continued to use the route to save fuel. That was despite two Ukrainian military aircraft having been shot down in the past week, and a third was damaged by a missile.
Immediately after yesterday's crash, four more civilian aircraft were believed to have followed the same path, including those of Singapore Airlines and Emirates.
But Qantas and South Korea's two main airlines, Korean Air and Asiana, said they had all re-routed flights in March.