In the study, carried out by researchers from Ben-Gurion University in Israel, participants looked at two versions of someone's face.
They found smiling people looked older because it made wrinkles around their eyes more obvious.
However, in a second experiment researchers were asked to recall the results and strangely remembered smiling faces as younger.
"In the experimental session, smiling faces were perceived as older than neutral faces", researchers wrote in the paper published in the April issue of Psychonomics Bulletin and Review.
"Nevertheless, after the experiment, consistent with their retrospective evaluations, participants recalled smiling faces as being younger than the neutral faces."
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Researchers think this is because humans are so conditioned to associated smiling with being youthful.
In a third experiment, researchers found surprised faces looked youngest of all.
"Smiling faces were again perceived as older than neutral faces, which were in turn perceived as older than surprised faces", researchers wrote.
"Again, retrospective evaluations were consistent with the belief that smiling makes people look younger.
They found people with genuine smiles, rather than simply looking cheerful or having a blank expression, were even more likely to be seen as healthy and glowing.
And the effect of having a positive expression on perceived health was as powerful as having the correct BMI, plastering on foundation, blusher and mascara or looking young rather than old, the study found.