"Of course you wear knickers under your pyjamas," she countered.
Constantine was taken aback by Barnett's vehement response and questioned if she was being honest.
"Do you wear knickers under your pyjamas when you go to bed? No that's not true, shut up!"
Barnett was emphatic. "Yes. You always need a gusset".
When a short clip was posted to social media, the responses flooded in.
Some argued that not wearing underwear meant changing pyjamas every night and that the freedom wasn't worth the extra laundry.
Others argued that the breeze downstairs was much-needed.
"'Never knickers under PJs nor a nightdress! Fresh air!" one person wrote.
Another argued that "air needs to circulate in your lady parts at night".
"I suppose they could prevent wedgies," one woman mused.
The debate made it to the BBC Woman's Hour broadcast itself, where listeners were keen to share their thoughts.