And Camilla's biography on the website and Buckingham Palace's page have also removed the statement.
But Clarence House said it was removed because the public was no longer interested in the issues.
A spokesman told The Sun: "This is one question Clarence House has not been asked for some time, which is why it no longer features."
However, Joe Little, of Majesty Magazine, said the removal was a "very interesting development".
He added: 'I'd be amazed if he didn't want her to be Queen. I very much hope she will be Queen Camilla.'
When asked in 2012 is the Duchess could be queen, Charles responded: 'We'll see. That could be.'