Footage shows the President and First Lady making their way down the plane's stairs, with Donald twice attempting to grab Melania's hand. However, both times, she pulls her hand away.
Melania, 50, was holding a handbag with her left hand, and she pulled it in closer to herself as he attempted to hold her hand.
Social media was abuzz over the apparent snub.
The President and First Lady disembark from Air Force One.
One Twitter user joked the First Lady is "voting for Biden", while another tweeted "Welcome to the resistance" on a post featuring the Air Force One footage.
"It looks like she needed her hand to hold down her dress as it was blowing up. After the stairs, she switched her hand holding the purse so she could hold his hand on the way to the helicopter," JJ tweeted.
It's not the first time the President has been snubbed when trying to hold the First Lady's hand.
In May 2017, Melania batted Trump's hand away after they landed in Tel Aviv, refusing to hold his hand as they greeted Israeli Prime Minister Benjamin Netanyahu and his wife, Sara Netanyahu.
The next day, the President was again rebuffed after the couple flew to Rome.
It even happened when Donald and Melania arrived in Washington on the eve of his inauguration in 2017.