Samantha claimed Meghan has fabricated stories of growing up in "virtual poverty" because her dad paid for her to attend dance and acting classes, and "elite and expensive private schools".
And despite the duchess' claims to Oprah and talk show host Ellen DeGeneres that she worked hard to put herself through university, Samantha insisted their father covered the costs of Meghan's tuition and living expenses at Northwestern University.
Samantha accused Meghan of lying about her and her childhood as part of a calculated effort so they "could not interfere with or contradict the false narrative and fairytale life story concocted by" the duchess.
Samantha has claimed her estranged relative's comments have damaged sales of her autobiography, "The Diary of Princess Pushy's Sister", prevented her from getting work, and caused emotional and mental distress.
A lawyer acting on behalf of the duchess - who has children Archie, 2, and Lili, 8 months, with Harry - branded the lawsuit "baseless and absurd".
Michael Kump said: "This baseless and absurd lawsuit is just a continuation of a pattern of disturbing behaviour. We will give it the minimum attention necessary, which is all it deserves."