Executive producer Chuck Lorre confirmed that, after 12 seasons and a record-breaking 279 episodes, The Big Bang Theory will air its final-ever episode in May 2019.
When it wraps, it will be the longest-running multi-camera series in television history.
After the news broke, Cuoco took to Instagram to thank the show's dedicated fans, admitting she was "drowning in tears".
Sharing a photo of herself with Johnny Galecki, Jim Parsons, Melissa Rauch, Kunal Nayyar, Mayim Bialik and Simon Helberg, she wrote: "This ride has been a dream come true and as life changing as it gets.
"No matter when it was going to end, my heart would have always been broken in two."
Parsons has not responded to the reports that he may be behind the show's ultimate demise, however he penned an emotional message about its end, sharing the below lengthy tribute with his 6.4 million Instagram followers.
In a joint statement confirming it is the end of the road for The Big Bang Theory, WBTV, CBS and Chuck Lorre Productions said: "We are forever grateful to our fans for their support of The Big Bang Theory during the past 12 seasons.
"We, along with the cast, writers and crew, are extremely appreciative of the show's success and aim to deliver a final season, and series finale, that will bring The Big Bang Theory to an epic creative close."