The comedian also took aim at SAS Australia, and Plate of Origin.
"SAS Australia was a huge success this year for Channel 7. Congratulations, they put celebrities through their paces.
"It was so entertaining that they almost forgot about the war crimes," Gleeson said.
"This year Channel 7 also poached Gary Mehigan and Matt Preston. They left MasterChef to launch Plate Of Origin, which I suspect completed its only season," he said about the low-rating show.
"Plate of Origin was a TV competition where Gary Mehigan and Matt Preston unknowingly competed to answer the question, 'Was MasterChef's ratings success due to the talent or the format?' Spoiler alert, it was the format."
The 2020 AACTAs were broken into two ceremonies due to Covid-19 – a film ceremony in the afternoon and a TV/online ceremony in the early evening.
Rove McManus and Susie Youssef hosted the afternoon film awards, with Tom Gleeson taking the reigns for the TV ceremony.
Guests who attended at least one of the ceremonies included Delta Goodrem, Tim Minchin, Matt Okine, Abe Forsythe, Eliza Scanlen, Anne Edmonds and Melissa Leong.