Though the N-word is used throughout the song by Burberry, it is the first time ever that Kylie has been heard saying it.
And the word is widely considered offensive for non African Americans to use because it was commonly used in the past as an ethnic slur by white people to identify people with black skin.
Beautiful Day was released on Wednesday and since news of Kylie's singing debut has spread, fans have taken to Twitter to complain about her contribution.
The backlash has prompted her collaborator, Jordyn, to defend the star on the micro blogging site and deny that Kylie said the controversial word.
When one Twitter user tweeted: "@KylieJenner Idc how famous you are, you ain't black & saying the n-word isn't funny or cool & that's not what u shld use ur celebrity for (sic)"
Jordyn snapped back: "Girl that's me and Justine. Kylie added one ad-lib. Lol... Why do some of y'all take life so seriously. Stop being mad at the world and take a joke... You hear us laughing in the song, clearly it was all of us together having fun (sic)"
- Bang! Showbiz