Oscar nominee Margot Robbie is expected to appear as Donna Freedman, and singer Delta Goodrem will also be back as Nina Tucker, a character she last played in 2002.
Goodrem has previously paid tribute to the show, saying on Instagram its cancellation was "truly the end of an era".
"[Neighbours] was a true school of the industry for so many people's paths, including mine!"
Guy Pearce also returned to Australia to film scenes as his former character, Mike Young, for the finale, according to Digital Spy.
"I'm an incredibly nostalgic person anyway so I've always wanted to step back onto the show at some stage and of course with the sad news that the show is finishing, it seemed like the appropriate time.
"Thankfully I was planning a trip back home anyway, so it just worked."
Decades of storylines
Neighbours has reached nearly 9000 episodes over the past 37 years and the residents of Ramsay St have certainly been busy during that time.
According to ABC News, the cast has clocked up 63 weddings, 20 births and 68 deaths, including shootings and a plane crash.
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