In the sultry black and white shot Gaga can be seen in a diner wearing a waitress outfit with towel slung over her shoulder.
Her short darkened locks are seen neatly styled and flipped over to reveal glamourous curls on the other side.
The 28-year-old pop diva plays Bertha, a waitress who tries to offer money to Joseph Gordon-Levitt's character, Johnny.
The full red band trailer was shown at last week's Comic-con convention in San Diego and shows the cast of anti-heroes up to their usual tricks.
Starring in the next installment is Juno Temple, Eva Green, Jessica Alba, Bruce Willis and Mickey Rourke among others.
The highly anticipated sequel will be released on August 22.
* Watch the trailer for Sin City 2 here (warning: explicit content).
- Daily Mail