In 2012 the property was given a capital valuation of $1.545 million, according to QV.
It was sold in 1991 - 24 years ago - for $400,000, and the Pitchfords bought it in 2007.
How the family ended up with such a menagerie of creatures great and small can be tied back to Mrs Pitchford's love of animals - they also have a dogs, cats and a parrot at their home in the Matamata town, where Dr Pitchford works.
The two buffalo - now about 1.5 tonnes each and too big for the pool - were acquired after Mrs Pitchford heard one was being "bullied" by an alpha male at another farm.
"Each animal has a personality of its own - Bassoon the bison is good friends with the two highland cattle," she said.
But at that hefty weight and nearly 2m high, "he's an animal to be respected", Dr Pitchford added.
Bayleys Matamata salesman Neville Jacques said the property had been a labour of love for its owners, who were reluctant to give it up - and their pets.
"They would love to see it passed on to someone equally as passionate about wildlife."
He said the property had a fully contained and insulated flat on site and could easily be made into a bed and breakfast to accommodate tourists visiting nearby Hobbiton.