K1W1 and Sparkbox, a specialist high-growth startup investment fund, sit alongside other investors in selecting and investing $18,000 in each start-up.
Teams then spend 12 weeks growing their business in the Wellington-based lab.
Applications are open, in return Lightning Lab will look for 8 per cent equity in the startups.
Each team works closely and intensively with Wellington-based lead mentors, and other national and international business experts are flown-in to offer advice.
Steve O'Connor, chief executive of startup and innovation centre Creative HQ, said the support meant teams would be exposed to world- class investors, and New Zealand's best business brains.
At the end of the intensive incubation period it is demo day, and teams will pitch their startups to a room filled with investors and market partners.
O'Connor said 50 per cent of accelerated startups were successful - a number he hoped to increase.
"The proof is in the pudding."