An OIO spokesman said none of the applications was declined.
Economic Development Minister Steven Joyce said the higher screening threshold was "not a particularly contentious issue" for New Zealand's negotiators when it was proposed by other TPP countries.
"There is no particular cost to New Zealand from an economic development perspective. It's good to encourage incoming investment and this reduces the red tape around that."
Mr Joyce said he had not been advised that the $100 million threshold had put off prospective investment. "But there's always a point at which it's not worthwhile for investors," he said.
Labour's finance spokesman Grant Robertson said his party had concerns about many of the OIO's processes, and would be concerned if the higher threshold increased the risk of bad investments.
New Zealand First leader Winston Peters said the threshold was already too low and the new policy would open New Zealand up to "dubious investment practices".
"At a time when huge sums of ill-gotten money are transferred around the world, and our checks through the Overseas Investment Office are already weak, we should be raising the bar against unscrupulous money merchants, not lowering it," Mr Peters said.
Mr Joyce disagreed, saying that money-laundering and anti-corruption systems were already in place.
"I don't think it creates any risks for New Zealand," he said.
A Government briefing paper said the TPP would not affect foreign purchases of sensitive land, which would continue to be screened to make sure they passed a "benefit to New Zealand" test.
New Zealand already has a higher threshold of $496 million for Australian investors as a result of its Closer Economic Ties agreement.
The Australian Government's threshold for screening foreign investments is $274 million, though the threshold is lower for some types of investment and there is a complete ban on sales of residential property to overseas buyers.