This isn't to say you were wrong to thank him, but a simple note - "I noticed what you did, and I appreciate it" - would have left you less invested in getting a response.
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Q: Is there a professional way to ask a colleague to wash their hands after using the bathroom? One individual in my office never does. Most of us in the office have witnessed it firsthand. It's gross and a great way to spread disease. We don't have an HR department, and asking the boss to address it isn't an option. Trying to avoid every door knob, copier, refrigerator door, etc., the person touches isn't realistic, either.
A: "Sweetie, did you wash your hands after going potty - Oh! I'm so sorry.We're toilet-training our toddler, so I'm in the habit of saying that every time I hear a flush."
Honestly, I don't have any magic words for calling out another adult for haphazard basic hygiene. You might put your mind at ease by placing sanitizing gel pumps and wipes in shared workspaces for everyone's use. That would also defend against the microbial menaces even your most fastidious colleagues have likely picked up from subway poles, or their shoes, or the change they received with their morning latte.