Green party co-leader Russel Norman said the announcement raised questions about motives.
"Is this part of a bigger game that Rio Tinto are playing in order to put pressure around the contract? Is it a shot across the bow of Meridian, saying we're serious this time?" Norman said.
"It says the threats around potential closure or significant reduction in demand are real."
Prime Minister John Key said the job losses were disappointing but a matter for the company.
"Unfortunately it reflects what is happening, which is international aluminium prices are weaker and the company is looking to cut its costs to maintain the rest of its operation in more difficult times."
Key said while it was well known Rio Tinto was seeking to sell the smelter, it remained an important asset to the company.
He indicated Rio Tinto would find it difficult to renegotiate short-term changes to what was a "pretty rock solid" contract for the next three years.
"It's a very well constructed contract from the Government's point of view."
He said he expected issues around the contract would be resolved.
General manager Ryan Cavanagh said 35 positions of the 100 had already gone through attrition.
TIWAI POINT SMELTER
* Employs 750 directly.
* Employs a further 3200 indirectly by company estimates.
* Contributes $500m to the Southland economy annually.