Spokesmen for Gates and Redmond, Washington-based Microsoft didn't respond to requests for comment.
Bill and Melinda Gates have given away about $35b (NZ$48) of stock and cash since 1994, based on the value of the shares at the time of gifts, according to a review of Gates Foundation tax returns, annual reports and regulatory filings.
Gates created the Giving Pledge in 2010 with billionaire investor Warren Buffett, and they have been joined by 168 others who promised to give the majority of their wealth to charity.
Gates probably has directed more than 700 million shares of Microsoft into the foundation, adjusting for stock splits, and he would be about $50b richer today had he kept them. The June gift represents 38 per cent of his holding in the company and is the latest in a long line of Microsoft share disposals that have whittled his stake from 24 per cent in 1996 to 1.3 per cent now.
Gates remains the richest person on earth after the donation with a fortune the Bloomberg Billionaires Index valued at $86b (NZ$119b) as of 10:40 a.m. Tuesday in New York. His donation once again puts Amazon.com founder Jeff Bezos close to the top spot, with a net worth of $84.5b (NZ$117b).
Bezos, 53, whose fortune has surged 30 per cent since Jan. 1, briefly leapfrogged Gates last month to become the world's richest person on an intraday basis.