Prince Harry arrived at Twickenham to lay a poppy wreath ahead of Remembrance Sunday - but there was no sign of his new girlfriend, Meghan Markle.
The actress, 35, has been staying with the prince in London, prompting fierce speculation that she would be accompanying him to this afternoon's game between England and South Africa.
However, when he arrived at the stadium in southwest London and placed a wreath of poppies outside the ground, she was nowhere to be seen.
Instead, the 32-year-old was sat next to Princess Charlene of Monaco, 38, during the Autumn International - which England won 37-21.
Bookies slashed their odds that the Prince and the US actress will be seen together at the 82,000-seater stadium to see the squad take on the Springboks after Ms Markle was spotted in Kensington on Thursday.
The pair are said to have been dating for several months but have not been seen in public together.
Harry recently confirmed they were an item and Ms Markle was spotted returning to Kensington Palace after a shopping trip to Whole Foods.
It had also been suggested that this weekend, Ms Markle could be welcomed into the heart of the Royal family this weekend, because most of its members are in the capital for a weekend of national commemorations to mark Remembrance Sunday.
Courtiers are suggesting Meghan could meet Prince Charles at Clarence House, while The Duke and Duchess of Cambridge are Harry's near neighbours at the palace.
The Prince took his last girlfriend Cressida Bonas, 27, to an England-Wales match in March 2014, sitting with her in the stands among other spectators.
He was photographed a number of times with long-term love Chelsy Davy, 31, at Twickenham watching the national side.
Harry waved to people inside the stadium on Saturday, before taking his seat next to Princess Charlene - a former Olympic swimmer for South Africa and the wife of Prince Albert II.
At the foot of the gates there are 15 roses which are copies of those which appeared on the 1914 England shirt - these give rise into poppies made from the brass shells fired by German artillery into the Allied trenches.
The royal was joined by Former England flanker Lewis Moody and RFU president Peter Bains.
Before taking his seat in the royal box, Harry met with Richard Slocock whose grandfather played for England and was killed at the Somme.
Harry's trip to Twickenham comes as steamy footage of Meghan Markle has re-emerged of her lying naked in bed.
The risque scene shows the actress in bed with co-star Patrick J Adams in series three of the hit American show Suits.