The contaminated seed, linked to Italian imports, was sown on more than 250 properties from Southland to Waikato.
Nationwide, velvetleaf plants were recovered from 251 properties, including 45 in Otago and 55 in Southland.
The Southland search alone cost about $700,000.
Mr Yard said plants undetected last season could lead to a "major infestation" this season.
"If left unmanaged, those plants will be a significant farm and biosecurity issue."
Farmers and contractors had to realise it was an offence under the Biosecurity Act 1993 to plant the six contaminated velvetleaf seed lines.