Mr Bodeker said his staff in Central Otago were liaising with the Queenstown Lakes District Council's compliance staff and Otago Fish & Game.
''So far, there is no obvious cause that anyone can point to.
''There is no construction site nearby and no signs of the sort of land disturbance or other activity that might generate pollutants.
''We are keeping an open mind about the cause at this stage and not ruling anything in or out.
''We are very keen to hear from anyone who has noticed any unusual activity along Mill Creek in the Speargrass Flat area.''
The regional council regularly monitors water quality in Mill Creek, as it is one of the significant inflows of Lake Hayes.
Mr Bodeker said calling the ORC's 24-hour pollution number - 0800 800 033 - was the easiest way for people to report water quality issues anywhere in Otago.