Rowe said the knowledge was being shared with the sheep industry in New Zealand, with researchers globally and was also assisting research into breeding lower methane-emitting cattle.
"Research like this is critical for the agriculture sector, which produces almost half of New Zealand's greenhouse gas emissions and needs practical tools to help achieve a reduction target of 24 per cent - 47 per cent less methane by 2050," she said.
Judges referenced the major significance of AgResearch's work within New Zealand and the "tangible contribution to the global issue of our time".
The world-first AgResearch achievement also featured engineering innovation in the form of specially designed portable accumulation chambers that can be taken on to farms to measure the methane emissions of individual sheep.
Rowe said it was humbling to have had her work and that of her colleagues recognised.
"It has taken well over a decade to reach this point and it has been a long journey by a dedicated team of scientists.
"We have worked side by side with sheep breeders and farmers to achieve a practical outcome that can be applied on farms.
"It is a fantastic feeling to know that we have achieved something that will make a difference for the livestock industry, for New Zealand, and the world when it comes to climate change.
"This is why we do our science - to make a real difference."
They had been fortunate to have great support from its funders in Pastoral Greenhouse Gas Research Consortium and the New Zealand Agricultural Greenhouse Gas Research Centre, and to be able to work closely with the sheep industry through Beef+Lamb Genetics.
"We think that when this low methane breeding trait is incorporated into New Zealand's entire sheep flock, the methane reduction could be in the area of 0.5 per cent to 1 per cent annually, which will be significant as it accumulates over time," she said.