Michael Burton from Hoverwing, Roger Martin from Happy Ewe Cycle Tours and Gary Coker from Canopy Tours are among the guides who have entered the competition.
The show's presenter Jo Holley will visit three guides in each of the 10 episodes, Mr Burton said.
"I think they are really going to get a lot from it," he said.
Guest judges will also rate the experience.
Rotorua Canopy Tours' Mr Coker said he did not know too much about the show, but assumed it would be a "day in the life of" sort of thing.
He said any increased awareness of the conservation projects at Canopy Tours would be beneficial.
Roger Martin from Happy Ewe tours said having national exposure would be beneficial for his business and all of Rotorua.
"Basically, it would be great. I would just take the show on the regular route and show them Rotorua."
-To see the guides' videos and find out how to vote for your favourite, go to www.facebook.com/topguidetv