She said it followed on from a march held in Rotorua in November, which attracted about 200 people.
"Last November 10,000 New Zealanders told Prime Minister John Key he has no right to make this deal in our name. He ignored us, so we are sending him an even stronger message on Saturday."
The theme of the national day of action is "TPPA: No Deal". Speakers include Green Party MP Catherine Delahunty, Rotorua-based NZ First MP Fletcher Tabuteau and Mana Party's Annette Sykes.
Rotorua's Potaua Baisiny-Tule from TangataWhenua.com will also speak.
Ms Delahunty will later speak at the Rotorua Arts Village on gold exploration.