Police said one person was transported by helicopter to Hawke's Bay Hospital in a serious condition.
A police spokeswoman said there were no blockages to the road.
The incident comes less than four months after a person died following a crash in Takapau in Central Hawke's Bay on July 30.
Another person was also seriously injured after a nose to tail crash on the Hawke's By Expressway on Sunday evening.
Emergency services were called to the two-vehicle crash in Waiohiki, Hastings, about 4.51pm on Sunday.
Nobody was trapped, but a St John Ambulance spokeswoman said one person in a serious condition was transported to Hawke's Bay Hospital.
Police said the southbound lane of the Hawke's Bay Expressway was blocked periodically.
Three fire trucks and two ambulances attended the scene.
