The aim is for everyone in port activities to have a better understanding of the biosecurity risks. The working group is aiming to strengthen the ministry's screening and inspection programmes.
Mr Guy said the importance placed on the initiative was reflected by the fact that Overseas Trade Minister and Rotorua MP Todd McClay also attended the Tauranga launch.
KVH chief executive Barry O'Neil said the port was a key area of risk for pest incursions.
"It's a growing port and an important port for the region and for our community. We fully support the port. What we don't want is that it creates a potential risk for our primary sector."
Zespri chief executive Lain Jager, who was one of about 40 industry and government leaders attending the launch, said the biosecurity initiative was a genuine example of government and local industry working together.
"And that's critical," said Mr Jager. "In 2010 we were heavily impacted by Psa. But a really important idea is that the biosecurity risk pressure hasn't diminished. There are many other pests and diseases that can come in. This is about protecting our future."
Standing guard:
* Some extra cruise ship biosecurity measures are already in place.
* Detector dogs have been introduced at major cruise ship ports over the past 12 months.