The Heartland team will play in the second-tier of the tournament for the Michael Jones Trophy at Owen Delany Park in Taupo.
Mark Rutene takes over as head coach after being assistant coach last year.
"There are at least seven or eight of the players who have played in the senior Heartland teams," he said.
"The tournament recognises the boys who remain playing for their Heartland unions, but they can still be a part of a New Zealand set up.
"However, the challenge is different from the other teams, as the squad is made up of at least one player from each Heartland union.
"It means we arrive on the Monday and then only have three training runs before we play our first game.
"Most of the other teams have been together for at least 6-8 weeks and have played half a dozen games."
At the first tournament in 2014, Heartland did very well by beating Tasman, losing a high scoring game to Southland, then comfortably accounting for Hawke's Bay.
The locals in that team were Zyon Hekenui (Ruapehu), Mitchell Overton (Taihape), James Brett (Hunterville) and Kameli Kuruyabaki (then Pirates, now Kaierau).
Last year the side was not as successful with defeats to Counties Manukau and Northland, but finished well with a win over Auckland B.
Taylor Transom (Taihape), Joe Edwards and Dominic Broadhead (both Kaierau) were in that team, while Hakaraia was selected but injured.
The 2016 edition of the tournament starts on September 15.