After the photos are uploaded, scientists can scan through the pictures, which, in rare cases, can lead to discoveries such as species living in a given area that had previously not been documented.
Department of Conservation senior ranger biodiversity Denise Fastier encouraged anyone to get out and have a go.
"You can even start in your own backyard and it is not restricted to native species."
Fastier said people didn't need to know what they were photographing because it can still help the scientific community.
The challenge runs from April 29 to May 2, during which people can upload their photos through the app.
Biodiversity Hawke's Bay general manager Debbie Monahan said Hamilton and the entire Hawke's Bay region were included in this year's challenge, as well as Auckland, Wellington, Christchurch and New Plymouth, plus many other cities around the world.
"The challenge is held over a weekend, giving families and children a great reason to get outside, connect with nature, and learn about the environment in which we live."
There are no prizes for taking part. More information at citynaturechallenge.org.