Gatland says the injuries are unfortunate but said the attrition of Super Rugby will always bring injuries.
"That's rugby. Everyone was fit and raring to go a few weeks ago and now we have some injuries but it gives a great opportunity for others to step up and get some experience," Gatland said.
Gatland expects the intensity to rise for the Chiefs this week despite the injuries but stressed his team would respect that Crusaders who come into the clash as three-time defending Super Rugby champions.
"We've got to give players in this squad some game time but we've also got to show the Crusaders some respect, they've earned that and we need to go out there and prove ourselves against what's undoubtedly been the best side in the competition for the past few years".
The full Chiefs squad to take on the Crusaders:
1. Aiden Ross
2. Samasoni Taukei'aho
3. Atu Moli
4.Tyler Ardron
5. Mitch Brown
6. Lachlan Boshier
7. Mitch Karpik
8. Sam Cane
9. Te Toiroa Tahuriorangi
10. Aaron Cruden
11. Solomon Alaimalo
12. Anton Lienert-Brown
13. Quinn Tupaea
14. Sean Wainui
15. Damian McKenzie
16. Billy Slater
17. Ross Geldenhuys
18. Ollie Norris
19. Naitoa Ah Kuoi
20. Pita Gus Sowakula