Tensions have again flared between Australian players and Virat Kohli after the Indian captain took a bye while Matthew Wade was down injured.

Wicketkeeper Wade appeared to hurt his arm after fumbling a Marcus Stoinis delivery during India's second one-day international win in Kolkata yesterday.

At the non-striker's end, Kohli took off on the quick run while Wade was hunched over.

It sparked Stoinis to confront Kohli at the end of the over and an angry response from Wade, who fumed at the batsman during the change of ends.

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The run-in continues a long list of unsavoury incidents involving the Australians and Kohli, who was on 79 at the time before top-scoring with a first-innings 92.

Kohli declared he will never be friends with any Australian player after this year's test series featured plenty of bad blood, but has since backed away from the comments.

But not every Australian was in Wade's corner, with former test fast bowler Stuart Clark saying the stoush was a distraction. "It's one run. Is this the biggest issue this team has got? If I'm Matthew Wade and I'm Marcus Stoinis - and full credit to him for standing up for Matthew Wade - I'd be worrying about my own cricket, rather than all this other stuff. This is piddly crap."

Wade appeared to struggle in the Indian heat in the day fielding session.

The innings was Kohli's first big score against Australia in any format since his match-winning 82no in the semifinals of the World Twenty20 in March last year.

The incident overshadowed the fact Kuldeep Yadav became the third India bowler to take a one-day international hat-trick as Australia were bowled out for 202 in a 50-run loss at Eden Gardens.

Yadav took 3-54 and his legspin partner Yuzvendra Chahal took 2-34, and India took a 2-0 lead in the five-match series.