The set-up phase is expected to begin on Monday next week and run until early December, when the store will open.
The Daily Post recently reported that 30 new staff were needed but that figure, which was provided by the company, was incorrect, a Mitre 10 spokesman said.
The Rotorua store is one of four Mitre 10 Mega stores opening in New Zealand between September and Christmas.
Mitre 10 chief executive John Hartmann said the company's growth was focused on rural and provincial centres, as well as in the main cities.
"It's good to be able to bring a significant new retail hub to a provincial heartland centre like Rotorua.
"We have a policy of actively engaging in our local community and the Bay of Plenty region will see our store become a focal point for community initiatives."
The store has already employed a general manager, trade manager and service and operation manager.
Positions for team supervisors in the garden, customer service and trade sections will be advertised this week as well as for trade representatives, a sales support administrator, store administrator and inwards goods, purchasing and stock control roles.
These staff will be part of the store set-up team that will take shape from Monday. Additional service assistants for the garden, trade, paint, kitchen and tool sections of the new stores will also be advertised in early October.
"Given the warm welcome and high degree of interest already shown in our plans for Rotorua we expect considerable interest in the recruiting process," Mr Hartmann said . "I think it is likely we'll need group interviews first before settling on shortlists for individual interviews to fill the roles."
The company had already been in touch with Work and Income to help identify prospective candidates.