After the driver replied "yes," Brown reached around the driver's plastic shield and dumped the liquid.
"I was provoked. I hate Metro," she said.
"I've been catching Metro for 35 years. They've never done s**t for me," the lifelong D.C. resident said.
"To say bizarre is really an understatement. It's a vulgar assault," said Metro Transit Police Chief Ron Pavlik.
A woman allegedly dumped a cup of fresh urine on a female bus driver after being told to 'have a nice day'. Photo / @Metrotransitpd / Twitter
Surveillance footage also captured Brown relieving herself in the back of the bus. She said she originally planned to throw the cup away, but then the driver was "rude".
"I had to go to the bathroom real, real bad. You know what I'm saying," Brown said to News4. "I just peed in a cup."
The driver went to a hospital for "decontamination," although she wasn't injured.
"I can't think of anything the operator did to warrant the assault," Pavlik said.
She acknowledged on Facebook that she was the woman who did it, even posting a link to a local news story about the incident and making a joke.
She posted on her Facebook page at 6.35am Wednesday morning that she was guilty.
"OK YALL KNOW IT WAS ME WHO DID THE BAD STUFF TO THE METRO OPERATOR!!!" she wrote.
"BUT I WANT TO APOLOGISE TO HER BUT METRO OWE ME AND SHE WAS BEING VERY RUDE. SO AT 2:40 AM I WALKED UP TO THE POLICE THAT WAS AT BENCO AND CONFESSED TO IT CUZ IT WAS ON MY HEART ALL DAY. BUT ANYWHO TRANSIT CAME. AND THEY WERE REALLY COOL I ALMOST FELT LIKE A CELEBRITY. IT TOOK ALL OF 20 MINUTES JUST GOTTA GO TO COURT FOR THE SIMPLE ASSULT."
Brown is a theological student and has three children and one grandchild.
The local Amalgamated Transit Union says Metro should do more to protect drivers/