Club president Paul Easton said it was a great cause and men had to look after themselves.
"It's more about awareness, getting everyone out there to get their check-ups when they need to."
Mr Easton said he had lost a close friend to cancer, and his father had come through prostate cancer. He said the reason his father survived was because his cancer was detected early.
Club captain Sean Strickett said a couple of his grandparents and an aunty had had cancer too.
They said about 20 club members were taking part, who had each picked a different style of moustache to attempt to grow.
Mr Easton said he thought he would go for a straight cut moustache while Mr Strickett said he might exaggerate the handlebars style.
Mr Easton said when you were clean shaven all year and then started to grow a moustache in November people noticed and asked about it.
"We don't make thousands and thousands but it's about the support."
They said they had a lot of support from Rotorua Club Incorporated, with a donation box on its bar.
Every Thursday the club also put a percentage of their entry fees towards the cause.
Anyone who would like to donate to the Rotorua Dart Club and support Movember can visit nz.movember.com, where they are registered under their club name.