One Twitter user wrote, "Your daily reminder that Martin Shkreli wants money so much he's depriving poor ppl of a cheap pill for a common AIDS complication."
John Carroll, the editor of Fierce Biotech, a daily newsletter about the industry, was one of the first to ask Shkreli directly to explain the move.
In a hot-headed Twitter exchange over the weekend, Shkreli declined to provide additional information and instead launched into a series of personal attacks against Carroll - calling him "irrelevant" and someone who doesn't "think logically."
Carroll tweeted, "Let's see if we can get a statement from @MartinShkreli. Martin, your co. just hiked the price of an old drug - new to you - by 5000%. Why?"
To which Shkreli responded, "@JohnCFierce You are such a moron."
Others also took to Twitter to express their outrage.
Celebrity blogger Perez Hilton wrote "I'm so disgusted that this hedge funder raised the price of an AIDS medication by 5000%!."
In response to some of the backlash, Shkreli said "Media: sorry, no more interviews. I have a busy and important job. I've said what I needed to and anyone interested can view those pieces."
This isn't Shkreli's first price-hiking biotech rodeo. A year ago as the CEO of Retrophin, his company acquired Thiola and planned to increase its price by over 20 times.