Jonathan Joseph is among four British and Irish Lions omitted from England's squad for their three-day training camp in Oxford.
George Kruis, Kyle Sinckler and James Haskell have also been left out of the 33-man group who will gather on Sunday despite touring New Zealand with Warren Gatland.
Denny Solomona is absent after being reprimanded for a late-night drinking session during the last England camp and there is still no place for in-form Bath winger Semesa Rokoduguni.
Harlequins' rising star Marcus Smith, 18, is the headline inclusion in the squad for the final get together before series against Australia, Argentina and Samoa.
"This camp in Oxford is a really positive opportunity for the group to come back together and sharpen our focus and preparation ahead of our first match against Argentina," coach Eddie Jones said.
England 33-man squad:
Forwards: Dan Cole, Tom Curry, Tom Dunn, Charlie Ewels, Ellis Genge, Jamie George, Dylan Hartley, Nathan Hughes, Nick Isiekwe, Maro Itoje, Joe Launchbury, Courtney Lawes, Joe Marler, Chris Robshaw, Sam Underhill, Harry Williams, Billy Vunipola, Mako Vunipola.
Backs: Mike Brown, Danny Care, Elliot Daly, Owen Farrell, George Ford, Piers Francis, Alex Lozowski, Jonny May, Jack Nowell, Henry Slade, Marcus Smith, Ben Te'o, Anthony Watson, Marland Yarde, Ben Youngs.